rabbit polyclonal anti tcf4 (Proteintech)

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Proteintech rabbit polyclonal anti tcf4
Rabbit Polyclonal Anti Tcf4, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+tcf4/TCF4+Antibody/pm42835275-143-32-35
Average 94 stars, based on 125 article reviews

rabbit polyclonal anti tcf4 - by Bioz Stars, 2026-10

94/100 stars

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Blocking Assay:

Article Title: USP30-AS1 Dictates Breast Cancer Cell Fate and Chemoresistance via a miR-3646/FZD7/Wnt/β-Catenin Circuit
Article Snippet: .. After blocking with 5% non-fat milk in TBST for 1 h at room temperature, the membranes were incubated overnight at 4 °C with the following primary antibodies: anti-TCF4 (13838-1-AP, Proteintech, Rosemont, IL, USA), anti-FZD7 (16974-1-AP, Proteintech), anti-AXIN2 (20540-1-AP, Proteintech), anti-CD44 (15675-1-AP, Proteintech), anti-P-gP (22336-1-AP, Proteintech), anti-c-MYC (10828-1-AP, Proteintech), anti-β-catenin (sc-7963, Santa Cruz Biotechnology, Dallas, TX, USA), anti-Cyclin D1 (#2978, Cell Signaling Technology, Danvers, MA, USA), and anti-GAPDH (#10442-1-AP, Proteintech). ..

Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells.
Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 ◦C with the following primary antibodies: anti-NANOG (bs0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1- AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).

Article Title: Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells
Article Snippet: .. After permeabilization and blocking with a solution containing 10% goat serum and 0.5% Triton X-100, the cells were incubated overnight at 4 °C with the following primary antibodies: anti-NANOG (bs-0829R, BIOSS, China), anti-SOX2 (11064-1-AP, Proteintech, USA), anti-OCT4 (SC-5279, Santa Cruz Biotechnology, USA), anti-TCF1 (14464-1-AP, Proteintech), anti-TCF3 (21242-1-AP, Proteintech), anti-TCF4 (13838-1-AP, Proteintech), anti-LEF1 (abs158608, Absin, Chian), anti-β-catenin (51067-2-AP, Proteintech). .. Subsequently, the cells were incubated for 1 h at room temperature with species-appropriate secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A21206, Invitrogen), F(ab’)2-Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 (A11017, Invitrogen).

Incubation:

Article Title: Tumor-derived exosomal miR-199b-5p promotes proliferation and epithelial-mesenchymal transition in non-small cell lung cancer by targeting CCNL1
Article Snippet: Subsequently, proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Millipore, USA) at a constant current of 300 mA for 90 min. .. The PVDF membrane was incubated overnight at 4 °C with the following primary antibodies: anti-CCNL1 (1:1000, cat. no. 13138–1-AP, Proteintech, Wuhan, China), anti-ARHGAP21 (1:1000, cat. no. PA5–100388, Thermo Fisher Scientific, USA), anti-EIF5B (1:1000, cat. no. 13527–1-AP, Proteintech, Wuhan, China), anti-TCF4 (1:1000, cat. no. 22337–1-AP, Proteintech, Wuhan, China), anti-GSK-3β (1:1000, cat. no. ab32391, Abcam, UK), anti-phosphorylated GSK-3β (p-GSK-3β, Ser9, 1:1000, cat. no. 5558, Cell Signaling Technology, USA), anti-GAPDH (1:10,000, cat. no. 10494–1-AP, Proteintech), and anti-α-tubulin (1:10,000, cat. no. 11224–1-AP, Proteintech). .. After washing the membrane with Tris-buffered saline with Tween 20 (TBST), it was incubated with a goat anti-rabbit IgG secondary antibody (1:10,000, cat. no. 20040, Absin, Shanghai, China) at room temperature for 1 h. Following additional washes with TBST, protein bands were visualized using an enhanced chemiluminescence (ECL) reagent kit (Proteintech, Wuhan, China), and images were captured using the SH-Advance523 imaging system (Shenhua Science Technology Co., Ltd., Hangzhou, China).

Membrane:

other:

Article Title: TIGAR maintains intestinal epithelial regeneration by stabilizing HMGCL and promoting β-catenin β-hydroxybutyrylation in burn-induced sepsis
Article Snippet: Anti-TIGAR (sc-166291) was purchased from Santa Cruz; Anti-TIGAR (22136-1-AP), Anti-HMGCL (16898-1-AP), anti-Park2 (14060-1-AP), anti-Tubulin (66031-1-Ig), anti-ubiquitin (10201-2-AP), anti-β-catenin (51067-2-AP), anti-TCF4 (22337-1-AP), anti-His-Tag (66005-1-Ig), anti-Flag-Tag (20543-1-AP, 66008-4-Ig), anti-HA-Tag (51064-2-AP), anti-PCNA (10205-2-AP), anti-FBL (16021-1-AP), anti-BrdU (66241-1-Ig), anti-Tom20 (11802-1-AP) were purchased from Proteintech (Wuhan, China); HRP-linked second Antibody (A0216, A0208) and anti-LGR5 (AF0165) was from Beyotime Biotechnology (Shanghai, China); anti-Ki67 (MA5-14520) was purchased from Invitrogen (California, USA); Anti-β-Hydroxybutyryllysine (PTM-1201RM) was purchased from PTM bio (Hangzhou, China); VeriBlot for IP Detection Reagent (HRP) (ab131366), Alexa Fluor® 488 Goat Anti-Rabbit IgG (ab150077) and Alexa Fluor® 488 Goat Anti-Mouse IgG (ab150113) were purchased from Abcam (UK); Cy3 Goat Anti-Rabbit IgG (SDI0012) and Cy3 Goat Anti-Mouse IgG (SDI0013) were purchased from SIDBIO (Chongqing, China); LPS (L9143) and CHX (239765) were purchased from Sigma-Aldrich (Saint Louis, Germany); (R)-3-Hydroxybutanoic acid sodium (HY-W015851), MG132 (HY-13259) and rapamycin (HY-10219) were purchased from MedChemExpress (New Jersey, USA); IntestiCult Organoid Growth Medium (Mouse, 06005) was purchased from STEMCELL Technologies (Vancouver, Canada).

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